Search

Home > Resources > GC-MS Facility > GC-MS instrument and charges
Reseources

Chromatography and Spectroscopy | GC-MS

UCT MCB Triple Quad GC-MS

GC is the preferred technique for the separation of low molecular weight metabolites which are either volatile or can be converted into volatile and thermally stable compounds through chemical derivatization before analysis. This includes primary metabolites, such as amino acids, amines, sugars, organic acids and fatty acids. GC is not the preferred method for secondary metabolites.
The coupling of GC to electron impact ionization (EI) MS is a hybrid technique in analytical chemistry and is considered to be one of the most robust and highly sensitive instrument platforms for metabolite analysis.
MCB’s Triple Quad GC MS is the ideal instrument to be used for compound target analysis. 

MCB Instruments
GC: Agilent 7890 (split inlet)
MS/MS: Agilent 7000C (electron ionization EI)
Carrier gas: Helium
Collison gas: Nitrogen

Columns
Agilent DB-5ms+DG, 30 meters, 0.250 mm (I.D.), 0.25 µm (film) up to 325°C (5% Phenyl-Arylene-95% methylpolysiloxane) + back flush column for non-polar & mixed-polarity compounds


Contact

For enquiries, please contant Pei-Yin Liebrich (pei-yin.liebrich@uct.ac.za).


User charges

Cost of GC use R250.00/hr
Setup of GC runs by MCB scientific officer R250.00/hr
Training / assistance / method development / derivatization / report R 250.00/hr
More than 10 hours 10% discount
   
Consumables  
Vials R8.00/vial
Vial caps R3.00/cap
Vial inserts (0.25 mL-tapered bottom)      R4.00/insert
Vial inserts (0.25 mL-with polymer feet)     R30.00/insert
Nylon hydrophillic syringe filter (0.22 µm 13 mm)     R6.00/filter
Syringe (1 mL)     R4.00/syringe
   
Chemicals  
FAMEs/d27-Myristic Acid Mixture (10 µl)     R100.00/10 µl
C8-C40 Alkanes (10 µl)     R30.00/10 µl
MSTFA (70 µl) R30.00/70 µl
   
External Academics     Total cost + 15% Vat
Industry Total cost + 30% +15% Vat