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Chromatography and Spectroscopy

GC-MS Instrument Details

Agilent Triple Quad GC-MS

The coupling of GC to electron impact ionization (EI) MS is a hybrid technique in analytical chemistry and is considered to be one of the most robust and highly sensitive instrument platforms for metabolite analysis and is referred to as the “gold standard” in metabolomics. The availability of both public and commercial EI spectral libraries facilitate the use of GC–MS as a metabolomics platform. GC is the preferred technique for the separation of low molecular weight metabolites which are either volatile or can be converted into volatile and thermally stable compounds through chemical derivatization before analysis. This includes primary metabolites, such as amino acids, amines, sugars, organic acids, fatty acids, long-chain alcohols, and sterols and is not the preferred method for secondary metabolites.

To quantify metabolites, either external calibration (which requires preparation of standard solutions) or internal calibration (based on the relation between the peak area of the compound and that of an internal standard) can be employed. 

MCB Instruments
GC: Agilent 7890 (Split or splitless inlet)
MS/MS: Agilent 7000C (electron ionization EI)
Carrier Gas: Helium
Collison Gas: Nitrogen

Columns
Agilent DB-5MS+DG, 30 meters, 0.250 mm (I.D.), 0.25 µm (film) up to 325°C (5% Phenyl-Arylene-95% methylpolysiloxane) – non-polar & mixed-polarity
SGE 25 meters, 0.32 mmm, 0.25 µm BPX70 (70% Cyanopropyl polysilphenylene-siloxane) – highly polar
 

Contact

For enquiries, please contant Pei-Yin Liebrich (pei-yin.liebrich@uct.ac.za).