Nikon Ti-E Inverted Microscope with Double Port FRET System
The MCB Microscope Facility houses a Nikon Ti-E inverted microscope with Z-stacking capability, and a motorized stage and CO2 incubator for live imaging of cells and FRET analysis. The Nikon Ti-E microscope is equipped with a top quality colour camera to capture bright field, phase contrast and fluorescent images from 4X to 100X magnification from live cells, as well as fixed samples. The microscope is also equipped with two, low light, cooled EMCCD (Electron Multiplying Charge Coupled Device) monochrome cameras for simultaneous detection of FRET signals. Phase contrast and Normarski DIC optics facilitate the acquisition of high contrast and high resolution images at low magnifications. Narrow band filter blocks allow fluorescent imaging in the UV, FITC and Texas Red range.
The microscope is linked to NIS-Elements AR imaging software which will allow image acquisition up to 6D (X, Y, Z, time, Lambda (wavelength), multipoint), archiving and analysis. This is particularly important for live cell imaging where information from all 6D are organized within one integral platform. Furthermore, the NIS-elements analysis software includes the capabilities for object counting, automated measurements, region of intent measurements and time measurements including image ratio ing of multi-channel images. The NIS Element software can also be used to stitch large images together seamlessly. Most importantly the NIS-Elements software has a built in image-database for the archiving of all image and meta-data captured on the microscope.
All images captured on the microscope will be stored on a dedicated 10 TB server (ROSALIND) managed by ICTS at UCT, from which users can download their images via the internet. All data stored on this dedicated server will be automatically backed up for 6 weeks from date of aquisition. The open source OMERO software will be used for users to view and manage their image data on the ROSALIND Data Store. This solution will be integrated into the University of Cape Town's long term data management strategy.