POLYMERASE CHAIN REACTION

Ed Rybicki

This is dealt with under the following subheadings:

• PCR PRIMER DESIGN AND REACTION OPTIMISATION
   
• STANDARD PCR PROTOCOL
   
• REVERSE TRANSCRIPTION PCR: RNA -> LOTS OF DNA
   
• CORE SAMPLE PCR
   
• CALCULATING CONCENTRATIONS FOR PCR
  

Lecture Material Available: Password protected, for UCT MCB3007A only

• Ed Rybicki: Introduction to PCR (2008)
• Ed Rybicki: PCR Primer Design (2008)
• Ed Rybicki: Other PCR Applications (2008)
• Fiona Tanzer: PCR Applications PowerPoint file
• mRNA Differential Display Acrobat (PDF) file
  

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Copyright: Ed Rybicki, 1996, 1998, 2005, 2006, 2008.