A Manual of Online Molecular Biology Techniques

by:

Professor Ed Rybicki
Dept Molecular & Cell Biology
University of Cape Town

copyright 2005

Contents:

• Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

• "Western" or Immunoelectroblotting of Proteins

• "Lunchbox" Immunoabsorption Techniques

• Affinity Purified Monospecific Antibodies to Gel-Purified Proteins

• Polymerase Chain Reaction 

  • PCR Primer Design and Reaction Optimisation
  • Standard PCR Protocol
  • Reverse Transcription PCR
  • Core Sample PCR (for bands in gels)
  • Calculating Concentrations for PCR Reactions

• Detection of Nucleic Acids by Hybridisation

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